Endocrine Abstracts

Objectives: The main goal of the present study is to investigate the role of N-linked oligosaccharides on the structure and function of human thyrotropin (hTSH). A second aim of the present study is to design new analogs of hTSH.Methods: Overlapping PCR technique was used to convert hTSH heterodimer to a biologically active single chain by fusing the α subunit to the carboxyl terminal end of hTSHβ subunit in the absence (hTSHβα) or pr...